| Sending insect specimens for identification |
Andras Szito, Taxonomist/curator
Many farmers, gardeners, Agency staff and members of the public require the identification of insects suspected of causing damage to agricultural crops, fruit trees or stock, or unusual insects, spiders and mites. Most of the insects are harmless, or even beneficial to man. For reliable identification the specimens should be undamaged and in an appropriate developmental stage.
Collection
Some of the insects show considerable variation in size, coloration and sculpture. With others, males and females cannot be separated by naked eye and for identification the entomologist needs both sexes. Sometimes the adult form is rare within the population. To overcome these difficulties always send 10-15 specimens. Make sure that the sample is always freshly collected because some insects deteriorate rapidly. The best means of preservation of specimens before correct killings is to keep the insects alive therefore, do not leave the sample in the car because the animals will die in a few hours.
Collection data
Adequate collection data are essential not only as an aid for the successful identification but also if the entomologist decide to keep the specimens in the Insect Reference Collection. A specimen without collection data is scientifically worthless and will not be kept in the Insect Reference Collection. Collection data should be always written by pencil, or pigment pen. Never use ball point pen, or any other pen.
The absolute minimum of information should be given is:
Additional valuable information:
Killing
If the specimens are predators, such as ground beetles, or plant feeders with strong jaws, such as scarab larvae, best to send dead because they may damage each other.
Most widely used killing agent and preservative is 70% alcohol, or methylated spirit. Both are very good for killing hard bodied insects. The small beetles, ants, bugs, aphids, thrips, spiders and mites can be killed this way. The great advantage is that it is easy to store and handle. Water at just below boiling point an excellent killing agent though it is not readily available on the field.
Butterflies and moths should be killed by freezing for 24 hours. In the field it may be impossible, in which case place them in airtight container with a ball of cottonwool, or tissue soaked in nailpolish remover, or acetone. If you using these chemicals, use glass jar for killing otherwise the chemicals may react with the plastic jar. After killing place them gently in a plastic container between layers of facial tissues.
Larvae, such as caterpillars, grubs and maggots should be killed by pouring on them just off boiling water. Few minutes later the specimens should be transferred into 70% alcohol if the specimens are not boiled first they will turn black in the alcohol and be very difficult to identify. Before killing the larvae, please, consult with the entomologist. He may decide the specimens should be sent alive for rearing to get the adult stage.
Insects which are attached to their host, such as scale insects, mealybugs, lerps are best to send alive on their host, in larger plastic container. Once their host is dry, (be careful do not let the plant become mouldy) the insect die but one can make perfect microscope slide mount from them.
There are many more killing methods that are widely used by specialist collectors and suit best to certain insect groups.
Dispatch
Use always the fastest available dispatch service.
Use always strong container/jar wrapped in bubble sheets, or at least send them in padded envelope. Unless you are sending live specimens, they should be sent in 70% alcohol, never in send in water. Soil living animals can be collected and sent in moist soil. The container should be topped up to minimise damage by shaking.
Do not post specimens in the second half of the week because they will likely remain in the post office over the weekend.
Necessary equipment for Field Officers:
10x magnifying glass
Pocketknife
Insect net
Round tipped forceps
Strong, sharp forceps
Small scissors
Small (0, or 00) brush
20 small 20-ml plastic vials
10 plastic specimen jars
5 cardboard specimen box
10 medium size plastic bag
10m medium size paper bag
200 ml 70 % denaturalised alcohol
50 ml AGA (for Agency staff available from Entomology Branch)
100 ml KAA (for Agency staff available from Entomology Branch)
Small pieces of acidfree cardboard
Available from:
Australian Entomological Supplies
PO Box 250
BANGALOW
NSW 2479
AUSTRALIA
Phone: 02 6684 7650
Fax: 02 6684 7188